In vitro anti onchocerca ochengi activities of extracts and chromatographic fractions of craterispermum laurinum and morinda lucida moses samje1,2, jonathan metuge2, james mbah3, brice nguesson3 and fidelis chongwa2 abstract background. This webapp provides a simple way to merge pdf files. A simple isothermal dna amplification method to screen. Onchocerciasis, or river blindness is a parasitic disease caused by the filarial worm onchocerca volvulus. Onchocerca ochengi microfilarial hamster model for onchocerciasis drug screens glory enjong mbah, rene bilingwe ayiseh and fidelis chongwa abstract background. The symbiotic role of wolbachia in onchocercidae and its impact on. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. Because of their predilection for host tissue sites, filariae are difficult to collect and their. How to merge pdfs and combine pdf files adobe acrobat dc. Onchocerca infected cattle produce strong antibody. Introduction introduction programme onchocercoses is funded by the deutsche forschungsgemeinschaft german research fund. Alternative treatment strategies to accelerate the.
Author summary filariae are predominantly tissuedwelling nematodes of the onchocercidae spirurida. Research open access discrimination between onchocerca volvulus and o. Onchocerca ochengi adult male amazon 20200426 20200426 tagged frontiers genomic epidemiology in filarial nematodes. The symbiotic role of wolbachia in onchocercidae and its. Development and validation of an onchocerca ochengi. Phytochemical analysis and in vitro anthelmintic activity. Approximately 17 million people are still infected with o. It contains one human parasite onchocerca volvulus which is responsible for the neglected disease onchocerciasis, also known as river blindness because the infected humans tend to live near rivers where host black flies live. Symptoms include severe itching, bumps under the skin, and blindness. The test is highly specific for the human parasite, as no crossreaction was detected using dna from the closely related and sympatric cattle parasite onchocerca ochengi. Summary epidemiological evidence has led to the hypothesis that the concurrent and predominant transmission of onchocerca ochengi by simulium damnosum s. They are parasites of terrestrial vertebrates and some of them are particularly well known as agents of human diseases in tropical environments e.
This free online tool allows to combine multiple pdf or image files into a single pdf document. Onchocerca ochengi, a bovine parasite of zebu cattle in africa is the closest related species to o. Onchocerca volvulus is the most pathogenic and is the agent of onchocerciasis or river blindness, a leading cause of morbidity and socioeconomic loss for the worlds poorest populations 1. Extracellular onchocercaderived small rnas in host. Animal onchocerciasis was assessed in nineteen cows from three.
The only drug currently used for the treatment of this disease and reduction of transmission is ivermectin. Onchocerciasis, caused by the parasitic nematode, onchocerca volvulus afflicts some 37 million people worldwide, and is the second leading infectious cause of blindness globally. Throughput is severely restricted by the logistics of identifying and experimenting on parasitized cattle in sub. Stagespecific proteomes from onchocerca ochengi, sister. Auranofin, approved for use in rheumatoid arthritis, killed both brugia spp. Onchocerca volvulus is a nematode that causes onchocerciasis river blindness, and is the second leading cause of blindness worldwide after trachoma. Onchocerca volvulus wikimili, the best wikipedia reader. Pdf merge combine pdf files free tool to merge pdf online. Onchocerca ochengi acquisition in zebu gudali cattle exposed to natural transmission. Soda pdf is the solution for users looking to merge multiple files into a single pdf document. Anthelmintic activity, acute toxicity of anacardium. Methodssmall rna libraries were prepared from total rna extracted from the nodule fluid of cattle infected with onchocerca ochengi as well as serum and plasma from humans infected with onchocerca volvulus in cameroon and ghana. The males are considerably smaller at around 4cm in length.
Plant was extracted in ethanol or methanolmethylene chloride. The results indicated that the assay is sensitive and rapid, capable of detecting dna equivalent to less than one microfilaria within 60 minutes. The biology of onchocerca ochengi, a filarial nematode. Over 40 million people are infected in africa, central america, and south america. Onchocerca volvulus is a nematode that causes onchocerciasis river blindness, and is the secondleading cause of blindness worldwide after trachoma. The filaria are a group of tissuedwelling parasitic nematodes of vertebrates that are spread by bloodfeeding arthropods. Onchocerca volvulus an overview sciencedirect topics. Onchocerciasis caused by onchocerca volvulus is the worlds second leading infectious cause of blindness. The disease was previously endemic in many countries. Easily combine multiple files into one pdf document. It is one of the 20 neglected tropical diseases listed by the world health organization, with elimination from certain countries expected by 2020. Onchocerca ochengi adult male amazon pixelmasterdesign.
Anthelmintic activity of phenolic acids from the axlewood. Immune recognition of excretory and secretory products of. Portions umbilical cattle skin with palpable nodules bought from local slaughterhouses were washed, drained and sterilized with 70% ethanol. Research open access discrimination between onchocerca. Two populations were observed between 2030 nt and 5070 nt figure 1, similar to the small rna profile previously observed for the in vitro secretion products of the gastrointestinal nematode h. The genome of onchocerca volvulus, agent of river blindness. The oil killed both the microfilariae and adult worms of o.
Onchocerciasis, also known as river blindness, is a disease caused by infection with the parasitic worm onchocerca volvulus. Endosymbiotic wolbachia bacteria in the worms are targets for a new therapeutic approach onchocerciasis, or river blindness, caused by the filaria onchocerca volvulus, affects more than 17 million people in africa, latin america, and yemen. Author summary onchocerciasis is a neglected tropical disease caused by the filarial nematode onchocerca volvulus. Our research network is based on more than 30 years of successful partnership of research institutes in germany and our partners based in cameroon.
Research open access a murine macrofilaricide preclinical. The filarid parasite onchocerca volvulus is the causative agent of human onchocerciasis river blindness, an infection characterized by chronic skin and eye lesions. Rna from the fluid of nodules of cattle infected with o. Anthelmintic activity of phenolic acids from the axlewood tree anogeissus leiocarpus on the filarial nematode onchocerca ochengi and drugresistant strains of the freeliving nematode caenorhabditis elegans volume 88 issue 4 d. Similarly, onchocerca ochengi females induce the formation of a nodule in the dermis of the bovine host, in which they remain sedentary and in close association with the hosts tissue. Onchocerca ochengi is a common filarial parasite in african zebu cattle, which is nonpathogenic, in contrast to its closest known relative onchocerca volvulus, which causes onchocerciasis river blindness in humans. Onchocerciasis is a vectorborne disease, where human beings are the only natural vertebrate host. Research open access extracellular onchocerca derived small rnas in host nodules and blood juan f quintana1, benjamin l makepeace2, simon a babayan3, alasdair ivens1, kenneth m pfarr4, mark blaxter1, alexander debrah5, samuel wanji6, henrietta f ngangyung7, germanus s bah7, vincent n tanya8, david w taylor2,9, achim hoerauf4 and amy h buck1 abstract.
You can either select the files you want to merge from you computer or drop them on. Research article open access onchocerca activity and. The microfilaricidal agent ivermectin is the principal means of controlling the disease, through mass treatment. However, this drug kills only the microfilarial stage of the parasite, and not the adult worm, and cases of parasite resistance to the drug have been recorded. Onchocerca ochengi is a noduleforming filarial nematode parasite of cattle in tropical africa and closely related to the human pathogen onchocerca volvulus. Only the hexane extract, an essential oil exhibited anti onchocerca activity. Attempts to eliminate onchocerciasis, primarily through the mass drug administration of ivermectin, remains challenging and has been heightened by the recent news that drugresistant. Onchocerca armillata is a parasite of the wall of the aorta of cattle, water buffaloes, goats, and camels, in south asia and equatorial africa. Onchocerca volvulus adults are coiled in fibrous tissue nodules in the subcutaneous tissue. Human onchocerciasis commonly known as river blindness is one of the most devastating yet neglected tropical diseases, leaving many millions in subsaharan africa blind andor with chronic disabilities. Here we identify extracellular, parasitederived small rnas associated with onchocerca species infecting cattle and humans. It is one of the twenty neglected tropical diseases listed by the world heath organisation who, with elimination from certain countries expected by 2020 john oneill, an irish surgeon, first described onchocerca volvulus in 1874 where he.
1586 1622 699 386 586 669 431 1054 1131 720 177 400 1564 925 817 1041 453 1063 1109 1612 1265 100 382 1049 62 1446 345 965 220 1510 1566 441 428 1155 1222 581 616 958 740 1148